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Detection of DNA of 'Candidatus Mycoplasma haemominutum' and Spiroplasma sp. in unfed ticks collected from vegetation in Japan OAK
Taroura, Shoko; Shimada, Yojiro; Sakata, Yoshimi; Miyama, Takako; Hiraoka, Hiroko; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽.
DNA fragments of 'Candidatus Mycoplasma haemominutum', a feline heamobartonella pathogen, were detected from unfed Ixodes ovatus collected from vegetation in Hokkaido, Fukushima and Yamaguchi Prefectures, and unfed Haemaphysalis flava in Yamaguchi Prefecture. This finding suggests that ixodid tick is a possible vector of 'C. Mycoplasma haemominutum'. Spiroplasma DNA was also detected from unfed I. ovatus in Hokkaido, Fukushima and Yamaguchi Prefectures. The analysis of nucleotides sequence suggested that this Spiroplasma was distinct from registered species.
Palavras-chave: Candidatus Mycoplasma haemominutum; Spiroplasma sp; Tick.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1654
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Molecular detection of hemoplasma infection among cats from São Luís island, Maranhão, Brazil BJM
Braga,M.S.C.O.; André,M.R.; Freschi,C.R.; Teixeira,M.C.A.; Machado,R.Z..
Hemoplasmas are bacteria that infect erythrocytes, attaching to the red blood cell. There is a need for more reports of hemoplasma infection prevalence and molecular characterization among cats in Brazil since there are only few published reports. The present work aimed to detect and molecularly characterize the presence of hemotrophic mycoplasmas in domestic cats with outdoor access from São Luís, Maranhão, Brazil. Twenty cats (10%) were positive for Candidatus M. haemominutum, five (2.5%) for M. haemofelis, and four (2.%) for M. turicensis based on 16S rRNA gene PCRs. Five cats (2.5%) were co-positive for Candidatus M. haemominutum and M. haemofelis. PCR diagnosis was confirmed by sequencing; and phylogenetic analysis was based on 16S rRNA and rnpb genes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cats; Mycoplasma haemofelis; Candidatus Mycoplasma haemominutum; Candidatus Mycoplasma turicensis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200018
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